Abstract
In this study the nucleotide sequence of the pncA gene from 59 Mycobacterium tuberculosis clinical isolates was analyzed. Mutations in the pncA gene were identified in 29 of 40 pyrazinamide-resistant isolates, and no pyrazinamidase activity was detected in 39 of them. Twelve mutations found in this work have not been described previously.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amidohydrolases / genetics*
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Antitubercular Agents / pharmacology*
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Drug Resistance, Bacterial / genetics*
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Microbial Sensitivity Tests
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Mutation*
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / enzymology
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Mycobacterium tuberculosis / genetics
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Pyrazinamide / pharmacology*
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Tuberculosis / microbiology
Substances
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Antitubercular Agents
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Pyrazinamide
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Amidohydrolases
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PncA protein, Mycobacterium tuberculosis