Purpose: To examine three layers of membranes detected by triamcinolone acetonide (TA) and indocyanine green (ICG) during surgery for macular hole and retinal detachment (MHRD) as a result of high myopia.
Design: Histologic study.
Methods: We excised three layers of membranes visualized with TA and ICG that were tightly adhering to the retinal surface during vitrectomy and examined them by transmission electron microscopy.
Results: The first membrane was made of acellular collagen fibers, suggestive of vitreous, and the second was comprised of parts of fibroblast-like cells and collagen fibrils, suggesting a proliferative epiretinal membrane. The third membrane was an internal limiting membrane.
Conclusions: Multiple components of epiretinal tissue could be delaminated with the assistance of TA and ICG during surgery for myopic MHRD.