Abstract
Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Compartmentation / physiology*
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Cell Fractionation / methods
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Cell Nucleus / chemistry
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Cell Nucleus / physiology
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Cells, Cultured
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Cytoplasm / chemistry
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Cytoplasm / physiology
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Disulfides / metabolism*
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Electrophysiology
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Epidermal Growth Factor / metabolism*
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / metabolism
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Humans
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Keratinocytes / cytology
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Keratinocytes / drug effects
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Membrane Proteins / metabolism
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Oxidation-Reduction
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Phosphorylation
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Reactive Oxygen Species / metabolism
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Signal Transduction / physiology*
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Sulfhydryl Compounds / metabolism*
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Thioredoxins / metabolism
Substances
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Disulfides
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Membrane Proteins
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Reactive Oxygen Species
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Sulfhydryl Compounds
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TXN protein, human
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Thioredoxins
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Epidermal Growth Factor
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ErbB Receptors