H9c2 cardiac muscle cells express all somatostatin receptor subtypes

R Granata; L Trovato; S Destefanis; F Settanni; E Ghigo

doi:10.1007/BF03346272

H9c2 cardiac muscle cells express all somatostatin receptor subtypes

J Endocrinol Invest. 2004 Oct;27(9):RC24-7. doi: 10.1007/BF03346272.

Authors

R Granata¹, L Trovato, S Destefanis, F Settanni, E Ghigo

Affiliation

¹ Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Turin, Turin, Italy.

PMID: 15648542
DOI: 10.1007/BF03346272

Abstract

The aim of the present study was to verify the hypothesis that SS receptor subtypes (SSTRs) are expressed by H9c2 cardiac muscle cells. SSTRs expression was investigated by RT-PCR and Western blot analysis at both mRNA and protein level. Our findings demonstrate that H9c2 cells express all SSTR subtypes I-5 (SSTRI-5) at the mRNA and protein level. Thus, H9c2 cells would represent a new model to study the direct biological activities of SS and its analogues at the cardiac level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Cell Line
Embryo, Mammalian
Fluorescent Antibody Technique
Myocytes, Cardiac / metabolism*
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA, Messenger / metabolism
Rats
Receptors, Somatostatin / genetics
Receptors, Somatostatin / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Staining and Labeling

Substances

Protein Isoforms
RNA, Messenger
Receptors, Somatostatin