Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea

Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):165-170. doi: 10.1099/ijs.0.63315-0.

Abstract

Three moderately halophilic, spore-forming strains, designated BH030062T, BH030049 and BH030080, were isolated from a solar saltern in Korea. Phylogenetic analyses and comparative 16S rRNA gene sequence studies revealed that the isolates represent a novel distinct monophyletic lineage within the phyletic group classically defined as the genus Bacillus and are most closely related to members of the genera Gracilibacillus (93.7-95.1 % similarity), Virgibacillus (93.5-94.8 %), Halobacillus (94.8-95.9 %), Filobacillus (94.4-94.8 %) and Lentibacillus (93.3-93.7 %). Strain BH030062T was strictly aerobic, rod-shaped, Gram-positive and motile by means of peritrichous flagella. It grew in the presence of 1-15 % (w/v) NaCl and at temperatures of 15-45 degrees C. The cell wall peptidoglycan contained A1gamma-meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(16 : 0). DNA G+C content was about 41 mol% and the major isoprenoid quinone was MK-7. On the basis of their physiological and molecular properties, the isolates represent a new genus, Pontibacillus gen. nov., and novel species, Pontibacillus chungwhensis sp. nov. The type strain is BH030062T (=KCTC 3890T=DSM 16287T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / classification*
  • Bacillaceae / genetics
  • Bacillaceae / isolation & purification
  • Bacillaceae / physiology
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Genes, rRNA
  • Korea
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sodium Chloride*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/AY553296
  • GENBANK/AY603361
  • GENBANK/AY603362