Abstract
Four strains isolated from Indian dairy products and initially identified as Lactobacillus delbrueckii could not be assigned to a definite subspecies because molecular identification and phenotypic traits did not agree with those of recognized subspecies of L. delbrueckii. Hybridization of total DNA (78-86 % against type strains of the other three subspecies), AFLP and RAPD-PCR fingerprints, phylogenetic analysis based on 16S rRNA gene sequences and sequence analysis of two coding genes (recA and hsp60), together with phenotypic profiles, indicated that the four strains form a coherent cluster and represent a novel subspecies, for which the name Lactobacillus delbrueckii subsp. indicus subsp. nov. is proposed. The type strain is NCC725T (=LMG 22083T=DSM 15996T).
MeSH terms
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Animals
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Chaperonin 60 / genetics
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DNA Fingerprinting / methods
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DNA, Bacterial / analysis
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Dairy Products / microbiology*
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Genes, rRNA
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India
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Lactobacillus delbrueckii / classification*
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Lactobacillus delbrueckii / genetics
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Lactobacillus delbrueckii / isolation & purification
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Phylogeny
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Polymerase Chain Reaction / methods
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 16S / genetics
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Rec A Recombinases / genetics
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Sequence Analysis, DNA
Substances
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Chaperonin 60
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DNA, Bacterial
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RNA, Ribosomal, 16S
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Rec A Recombinases
Associated data
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GENBANK/AJ586863
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GENBANK/AJ586864
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GENBANK/AJ586865
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GENBANK/AJ586866
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GENBANK/AJ586867
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GENBANK/AJ586868
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GENBANK/AJ586869
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GENBANK/AJ586870
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GENBANK/AJ586871
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GENBANK/AJ586872