Cell-permeable GPNA with appropriate backbone stereochemistry and spacing binds sequence-specifically to RNA

Anca Dragulescu-Andrasi; Peng Zhou; Gaofei He; Danith H Ly

doi:10.1039/b412522c

Cell-permeable GPNA with appropriate backbone stereochemistry and spacing binds sequence-specifically to RNA

Chem Commun (Camb). 2005 Jan 14:(2):244-6. doi: 10.1039/b412522c. Epub 2004 Dec 2.

Authors

Anca Dragulescu-Andrasi¹, Peng Zhou, Gaofei He, Danith H Ly

Affiliation

¹ Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.

PMID: 15724200
DOI: 10.1039/b412522c

Abstract

Guanidine-based peptide nucleic acid (GPNA) with a d-backbone configuration and alternate spacing binds sequence-specifically to RNA and is readily taken up by both human somatic and embryonic stem (ES) cells.

MeSH terms

Base Sequence
Cell Membrane Permeability
DNA / chemistry
Dipeptides / chemistry*
Dipeptides / metabolism*
Gene Silencing
HeLa Cells
Humans
Oligomycins
RNA / genetics*
RNA / metabolism*
Stem Cells / metabolism
Substrate Specificity

Substances

Dipeptides
Oligomycins
glutarylphenylalanine-4-nitroanilide
RNA
DNA