Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture

Dirk S Luijt; Petra A J Bos; Anton A van Zwet; Pieter C van Voorst Vader; Jurjen Schirm

doi:10.1128/JCM.43.3.1445-1447.2005

Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture

J Clin Microbiol. 2005 Mar;43(3):1445-7. doi: 10.1128/JCM.43.3.1445-1447.2005.

Authors

Dirk S Luijt¹, Petra A J Bos, Anton A van Zwet, Pieter C van Voorst Vader, Jurjen Schirm

Affiliation

¹ Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands. j.schirm@slgd.nl.

Abstract

A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.

Publication types

Comparative Study

MeSH terms

Culture Media
Neisseria gonorrhoeae / genetics*
Neisseria gonorrhoeae / isolation & purification
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics*
Sensitivity and Specificity

Substances

Culture Media
RNA, Ribosomal, 16S