Acceptable performance of VITEK 2 system to detect extended-spectrum beta-lactamases in clinical isolates of Escherichia coli: a comparative study of phenotypic commercial methods and NCCLS guidelines

Antonio Sorlózano; José Gutiérrez; Gonzalo Piédrola; María José Soto

doi:10.1016/j.diagmicrobio.2004.11.001

Acceptable performance of VITEK 2 system to detect extended-spectrum beta-lactamases in clinical isolates of Escherichia coli: a comparative study of phenotypic commercial methods and NCCLS guidelines

Diagn Microbiol Infect Dis. 2005 Mar;51(3):191-3. doi: 10.1016/j.diagmicrobio.2004.11.001.

Authors

Antonio Sorlózano¹, José Gutiérrez, Gonzalo Piédrola, María José Soto

Affiliation

¹ Department of Microbiology, School of Medicine, San Cecilio University Hospital, University of Granada, E-18012 Granada, Spain.

PMID: 15766605
DOI: 10.1016/j.diagmicrobio.2004.11.001

Abstract

The disk approximation method, Etest (AB Biodisk, Solna, Sweden), and VITEK 2 system (bioMérieux, Marcy l'Etoile, France) were used to study 399 extended-spectrum beta-lactamase (ESBL)-producing (115 strains) and non-ESBL-producing (284 strains) clinical isolates of Escherichia coli after recommended procedures. Comparative study of the phenotypic findings yielded data on the sensitivity, specificity, and positive and negative predictive values and performance of each method. The sensitivity (100% using 2 substrates), specificity (99.3-100%), and predictive values of the disk approximation, Etest, and VITEK 2 methods were similar.

Publication types

Comparative Study

MeSH terms

Bacteriological Techniques / methods*
Escherichia coli / enzymology*
Escherichia coli / genetics
Phenotype
Sensitivity and Specificity
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

beta-Lactamases