Brucella spp. lumazine synthase: a novel antigen delivery system

Vaccine. 2005 Apr 15;23(21):2784-90. doi: 10.1016/j.vaccine.2004.11.043.

Abstract

Lumazine synthase from Brucella spp. (BLS) was evaluated as a protein carrier to improve antigen delivery of KETc1, one of the peptides of the anti-cysticercosis vaccine. KETc1 becomes antigenic, preserved its immunogenicity and its protective capacity when expressed as a recombinant chimeric protein using Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2(d), H-2(k) nor H-2(b) haplotype-restricted albeit KETc1 is preferentially presented in the H-2(b) haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / immunology*
  • Brucella / enzymology*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Multienzyme Complexes / immunology*
  • Recombinant Fusion Proteins / immunology
  • Taenia solium / immunology*
  • Tetanus Toxoid / immunology
  • Vaccines, Synthetic / immunology*

Substances

  • Antigens, Helminth
  • Multienzyme Complexes
  • Recombinant Fusion Proteins
  • Tetanus Toxoid
  • Vaccines, Synthetic
  • 6,7-dimethyl-8-ribityllumazine synthase