Abstract
Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Algorithms
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Animals
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Biophysics / methods
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Cell Line
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Cricetinae
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Diffusion
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Green Fluorescent Proteins / metabolism
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Image Interpretation, Computer-Assisted / methods*
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Image Processing, Computer-Assisted / methods*
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Lasers
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Microscopy, Confocal / methods*
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Microscopy, Fluorescence / methods*
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Models, Statistical
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Normal Distribution
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Photons
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Time Factors
Substances
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Green Fluorescent Proteins