Enzymatic synthesis of oligosaccharides from branched cyclodextrins

Christel T Jørgensen; Allan Svendsen; Jesper Brask

doi:10.1016/j.carres.2005.02.004

Enzymatic synthesis of oligosaccharides from branched cyclodextrins

Carbohydr Res. 2005 May 2;340(6):1233-7. doi: 10.1016/j.carres.2005.02.004.

Authors

Christel T Jørgensen¹, Allan Svendsen, Jesper Brask

Affiliation

¹ Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark.

PMID: 15797141
DOI: 10.1016/j.carres.2005.02.004

Abstract

6''-alpha-Maltosyl-maltotriose and 6''-alpha-D-glucosyl-maltotriose were prepared from Novamyl degradation of 6-O-alpha-maltosyl-alpha-cyclodextrin and 6-O-alpha-D-glucosyl-alpha-cyclodextrin, respectively. NMR spectroscopy was used to elucidate their structural identities, in a combination of COSY experiments. Further, a mechanism for the degradation was proposed based on the Novamyl active site geometry.

MeSH terms

Carbohydrate Sequence
Cyclodextrins / chemistry*
Cyclodextrins / metabolism*
Enzymes / metabolism*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Oligosaccharides / biosynthesis*
Oligosaccharides / metabolism*

Substances

Cyclodextrins
Enzymes
Oligosaccharides