Methods to improve the yield and quality of DNA from dried and processed figs

Biotechnol Prog. 2005 Mar-Apr;21(2):546-9. doi: 10.1021/bp049710p.

Abstract

We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers
  • DNA, Plant / isolation & purification*
  • Electrophoresis, Agar Gel
  • Ficus / genetics*
  • Polymerase Chain Reaction

Substances

  • DNA Primers
  • DNA, Plant