The near infrared surface-enhanced Raman spectra (NIR-SERS) of amsacrine,an anticancer agent, on silver collids were recored with high quality. The NIR-SERS enhancement factor is up to 10(4) magnitude. In comparison with the near infrared Raman (NIR-Raman) spectra of solid amsacrine, more information of vibrational structures of amsacrine molecules at low concentration and almost in its practical application envi ronment, can be given by the NIR-SERS. The enhancement mechanism is associated with the adsorption of acridyl ring and sulfonyl on silver surface.