Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

J Clin Microbiol. 2005 Apr;43(4):2004-8. doi: 10.1128/JCM.43.4.2004-2008.2005.

Abstract

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques
  • Blood / microbiology
  • Cerebrospinal Fluid / microbiology
  • Fatal Outcome
  • Humans
  • Ill-Housed Persons*
  • Male
  • Meningitis, Pneumococcal / microbiology*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA*
  • Streptococcus pneumoniae / classification*
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification
  • Streptolysins / genetics*

Substances

  • Bacterial Proteins
  • Streptolysins
  • plY protein, Streptococcus pneumoniae