Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc

Michelle Kilcoyne; Alexander S Shashkov; Andrei V Perepelov; Evgeny L Nazarenko; Raisa P Gorshkova; Elena P Ivanova; Göran Widmalm; Angela V Savage

doi:10.1016/j.carres.2005.01.040

Structure of the O-specific polysaccharide from Shewanella japonica type strain KMM 3299T containing the rare amino sugar Fuc4NAc

Carbohydr Res. 2005 Jun 13;340(8):1557-61. doi: 10.1016/j.carres.2005.01.040.

Authors

Michelle Kilcoyne¹, Alexander S Shashkov, Andrei V Perepelov, Evgeny L Nazarenko, Raisa P Gorshkova, Elena P Ivanova, Göran Widmalm, Angela V Savage

Affiliation

¹ Department of Chemistry, National University of Ireland, Galway.

PMID: 15882857
DOI: 10.1016/j.carres.2005.01.040

Abstract

An acidic O-specific polysaccharide (PS) of the agar-digesting bacterium Shewanella japonica with the type strain KMM 3299(T) was obtained by mild acid hydrolysis of the lipopolysaccharide. The polysaccharide was studied by component analysis, methylation analysis, (1)H and (13)C NMR spectroscopy, including 2D NMR experiments. The PS was determined to have the following structure involving three unusual amino sugars:

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Fucose / analogs & derivatives*
Fucose / analysis
Fucose / chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
Shewanella / chemistry*
Shewanella / classification*

Substances

4-acetamido-4,6-dideoxygalactose
O Antigens
Fucose