Proteins, chlorophylls and lipids: X-ray analysis of a three-way relationship

Trends Plant Sci. 2005 Jun;10(6):275-82. doi: 10.1016/j.tplants.2005.04.007.

Abstract

Photosynthetic reaction centres and light harvesting complexes have been at the forefront of crystallographic studies of integral membrane proteins. In recent years, there have been spectacular advances in our understanding of the structure of (bacterio)chlorophyll-containing membrane proteins from oxygenic and anoxygenic phototrophs. In these complex structures, the protein scaffold encases different combinations of cofactors and interacts with several tightly bound lipid species that play a variety of hitherto unrecognized structural roles. Some of these lipids have relevance to the physiological function of the protein, whereas others are important for the formation of highly ordered crystals. The first site-directed mutagenesis studies of individual lipid binding sites have now underlined the importance of the lipid component for the structural stability of protein-cofactor-lipid complexes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / physiology
  • Chlorophyll / chemistry
  • Chlorophyll / physiology*
  • Crystallography, X-Ray
  • Light-Harvesting Protein Complexes / chemistry
  • Light-Harvesting Protein Complexes / physiology
  • Lipids / chemistry
  • Lipids / physiology*
  • Models, Molecular
  • Photosystem I Protein Complex / chemistry
  • Photosystem I Protein Complex / genetics
  • Photosystem I Protein Complex / physiology
  • Plant Proteins / chemistry
  • Plant Proteins / physiology*
  • Protein Conformation

Substances

  • Bacterial Proteins
  • Light-Harvesting Protein Complexes
  • Lipids
  • Photosystem I Protein Complex
  • Plant Proteins
  • Chlorophyll