Enteropathogenic Escherichia coli strains carrying genes encoding the PER-2 and TEM-116 extended-spectrum beta-lactamases isolated from children with diarrhea in Uruguay

J Clin Microbiol. 2005 Jun;43(6):2940-3. doi: 10.1128/JCM.43.6.2940-2943.2005.

Abstract

We studied 13 extended-spectrum beta-lactamase (ESBL)-producing enteropathogenic Escherichia coli isolates from children suffering acute diarrhea in Uruguay. ESBL characterization in crude extracts showed a single band at pI 5.4. PCR amplification and sequencing data allowed identification of blaPER-2 and blaTEM-116. Retrospective analysis suggests that these strains were disseminated in the community, even if unnoticed, prior to their access to the hospital environment more than a decade ago.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Cephalosporin Resistance
  • Child, Preschool
  • Diarrhea / epidemiology*
  • Diarrhea / microbiology*
  • Escherichia coli / drug effects*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification*
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism
  • Humans
  • Infant
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA
  • Uruguay / epidemiology
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Escherichia coli Proteins
  • beta-Lactamases
  • beta-lactamase TEM-103, E coli

Associated data

  • GENBANK/AJ786366
  • GENBANK/AJ847362
  • GENBANK/AJ847363
  • GENBANK/AJ847364