Structural basis of interaction between protein tyrosine phosphatase PCP-2 and beta-catenin

Sci China C Life Sci. 2005 Apr;48(2):163-7. doi: 10.1007/BF02879669.

Abstract

PCP-2 is a member of receptor-like protein tyrosine phosphatase of the MAM domain family. To investigate which part of PCP-2 was involved in its interaction with beta-catenin, we constructed various deletion mutants of PCP-2. These PCP-2 mutants and wild-type PCP-2 were co-transfected into BHK-21 cells with beta-catenin individually. An in vivo binding assay revealed that the expression of wild-type PCP-2, PCP-2 deltaC1C2 (deleted PCP-2 without both PTP domains) and PCP-2 deltaC2 (deleted PCP-2 without the second PTP domain) could be immunoprecipitated by anti-catenin antibody in every co-transfection, but PCP-2 EXT (deleted PCP-2 without the juxtamembrane region and both PTP domains) was missing, which implied that PCP-2 and beta-catenin could associate directly and the juxtamembrane region in PCP-2 was sufficient for the process.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cricetinae
  • Cytoskeletal Proteins / chemistry*
  • Cytoskeletal Proteins / metabolism*
  • Gene Deletion
  • Gene Expression
  • Precipitin Tests
  • Protein Tyrosine Phosphatases / analysis
  • Protein Tyrosine Phosphatases / chemistry*
  • Protein Tyrosine Phosphatases / genetics
  • Protein Tyrosine Phosphatases / metabolism*
  • Trans-Activators / chemistry*
  • Trans-Activators / metabolism*
  • Transfection
  • beta Catenin

Substances

  • Cytoskeletal Proteins
  • Trans-Activators
  • beta Catenin
  • Protein Tyrosine Phosphatases