Objective: To locate the region responsible for nuclear localization of protein sAC.
Methods: The eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.
Results: For some mutants, the localization of sAC changed. Deletion of some region made it unable to locate in the nuclear.
Conclusion: It is possible to figure out that the nucleotide region (739-1038 and 1045-1261) take charge of nuclear localization of sAC.