Human papillomavirus DNA detection in women with primary abnormal cytology of the cervix: prevalence and distribution of HPV genotypes

Cytopathology. 2005 Aug;16(4):199-205. doi: 10.1111/j.1365-2303.2005.00266.x.

Abstract

Objectives: In this study, we focus on the prevalence and occurrence of different anogenital human papillomavirus (HPV) genotypes in a first abnormal cervical screening test, and correlate HPV genotyping with the cytological diagnosis on thin-layer liquid-based preparations in routine gynaecological screening.

Methods: Out of 780 abnormal smears, 513 tested positive for HPV. All 25 different HPV types were identified by Line Probe Assay.

Results: The prevalence of high-risk HPV types increased from 72% in atypical squamous cell of undetermined significance to 94.5% in high-grade intra-epithelial lesion (HSIL). Co-infection with multiple HPV types was predominantly found in HSIL (35.8%). In the HSIL group the most common HPV types were 16, 52, 51 and 31; type 18 was rarely present.

Conclusion: The role of types 31, 51 and 52 should be considered in future studies on vaccine development.

MeSH terms

  • Adolescent
  • Adult
  • Age Distribution
  • Aged
  • Aged, 80 and over
  • Belgium / epidemiology
  • Cervix Uteri / pathology*
  • Cervix Uteri / virology*
  • Comorbidity
  • Cytodiagnosis
  • DNA, Viral / analysis*
  • Female
  • Genotype
  • Humans
  • Mass Screening / methods
  • Middle Aged
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / virology
  • Prevalence
  • Uterine Cervical Neoplasms / diagnosis*
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / virology

Substances

  • DNA, Viral