Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals

Hanneke Schuitemaker; Neeltje A Kootstra

doi:10.1385/1-59259-907-9:017

Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals

Methods Mol Biol. 2005:304:17-24. doi: 10.1385/1-59259-907-9:017.

Authors

Hanneke Schuitemaker¹, Neeltje A Kootstra

Affiliation

¹ Department of Clinical Vitro-Immunology, Sanquin Research at CLB, Academical Medical Center, Amsterdam, The Netherlands.

PMID: 16061963
DOI: 10.1385/1-59259-907-9:017

Abstract

HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.

MeSH terms

HIV Infections / blood*
HIV Infections / virology*
HIV-1 / isolation & purification*
HIV-1 / pathogenicity
HIV-1 / physiology
Humans
Neutralization Tests / methods
Viral Plaque Assay / methods
Virus Cultivation / methods*
Virus Replication / physiology*