Purpose: To study vitreous cavity humidity during fluid-air exchange in pars plana vitrectomy.
Methods: Intraocular humidity in the vitreous cavity was recorded for 2 min in six artificial eyes, six enucleated pig eyes, and ten patient eyes, after the eyes had been filled with either humidified air (75% humidity) or dry air (8% humidity).
Results: In artificial eyes the humidity levelled off at a value that was approximately equal to the humidity of the infused air, i.e., a mean of 71.9% when humidified air was used and a mean of 14.4% when dry air was used. In enucleated pig eyes humidity increased slightly with humidified air and remained stable with dry air. In patients intraocular humidity increased to over 90%, regardless of whether humidified or dry air was used.
Conclusion: In the living eye, dry air deprives the retinal tissue of humidity, which is lost into the vitreous cavity. This effect can be reduced by using humidified air.