Objective: To investigate the targeted killing effect of mucin-1 single chain antibody targeting, lentivirus-mediated suicide gene therapy and ganciclovir(GCV) in mucin-1+ ovarian epithelial carcinoma cells.
Methods: Mucin-1 single chain antibody targeting lentivirus produced by packaging cell line 293T transduced herpes simplex virus thymidine kinase (HSV-tk) gene into the ovarian epithelial cancer cell line SKOV3 (MUC1+). The infection effect was observed through fluorescence microscopy. Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) were resorted to demonstrate the successful transduction and transcription of the HSV-tk gene. After administration of GCV, changes of those cells were observed through optical microscopy. The cytotoxicity efficacy of HSV-tk/GCV system was evaluated by methyl thiazolyl tetrazolium method.
Results: It was observed through fluorescence microscopy that anti-MUC1 directed lentivirus can specificly infect MUC1+ ovarian cancer cells. A fragment of 600 bp was generated through PCR and RT-PCR which indicated successful transduction and transcription of the HSV-tk gene in SKOV3 cells. Changes of cells followed by administration of GCV were observed with optical microscopy. Significant cytotoxicity efficacy of GCV to SKOV3 was observed.
Conclusions: The HSV-tk gene can be targetedly transducted into MUC1+ ovarian cancer cell line under the mediation of anti-MUC1 directed lentivirus, and such HSV-tk/GCV system has targetingly killing effect on MUC1+ cancer cells.