Abstract
The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-alpha subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern / methods
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Cell Line, Tumor
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Cloning, Molecular / methods
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DNA, Complementary / genetics*
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Muscle, Skeletal / metabolism*
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Myosarcoma
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / metabolism
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Receptors, Cholinergic / classification
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Receptors, Cholinergic / genetics*
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Reverse Transcriptase Polymerase Chain Reaction / methods
Substances
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DNA, Complementary
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Protein Isoforms
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RNA, Messenger
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Receptors, Cholinergic