Fine needle aspiration of the seminal vesicles under transrectal ultrasound guidance was performed in a man with primary anorgasmy. The procedure included local anesthesia to the apex of the prostate and a 21-gauge needle for aspiration. A total of 3 mL of seminal fluid could be aspirated. Semen analysis showed a sperm count of 40 million/mL and 2% motility. Intracytoplasmic sperm injection was performed, pregnancy resulted, and a healthy girl was born. This is the first successful report of fine needle aspiration of the seminal vesicles in men whose infertility is caused by ejaculatory disorders or distal obstructive azoospermia. This finding could lead to an expansion of our armamentarium in assisted reproductive technology.