Formate dehydrogenase gene of Arabidopsis thaliana is induced by formaldehyde and not by formic acid

E Fukusaki; T Ikeda; T Shiraishi; T Nishikawa; A Kobayashi

doi:10.1263/jbb.90.691

Formate dehydrogenase gene of Arabidopsis thaliana is induced by formaldehyde and not by formic acid

J Biosci Bioeng. 2000;90(6):691-3. doi: 10.1263/jbb.90.691.

Authors

E Fukusaki¹, T Ikeda, T Shiraishi, T Nishikawa, A Kobayashi

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Japan.

PMID: 16232936
DOI: 10.1263/jbb.90.691

Abstract

Variability of expression of formate dehydrogenase (FDH) caused by uptake of C-1 compounds was examined by using Arabidopsis thaliana as a model plant. Effects of uptake of several C-1 compounds were evaluated by Northern blot analysis using cDNA of A. thaliana FDH prepared by cloning on the basis of known sequence. As a result, expression of the FDH gene in A. thaliana was not intensely influenced by formic acid, an inherent substrate for FDH, but strongly induced by its reduced form, formaldehyde.