Efficient solid-phase synthesis of trifunctional probes and their application to the detection of carbohydrate-binding proteins

Org Lett. 2005 Nov 24;7(24):5477-80. doi: 10.1021/ol0523188.

Abstract

[structure: see text] An efficient solid-phase synthesis of trifunctional probes containing a photoreactive group, a reporter tag, and a carbohydrate ligand was developed. Labeling studies with these probes demonstrate that specific lectins can be labeled with high sensitivity and selectivity. This technique serves as a powerful tool for the rapid detection and profiling of lectins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / chemistry*
  • Lectins / analysis*
  • Lectins / chemistry
  • Ligands
  • Molecular Probes / chemical synthesis*
  • Molecular Structure

Substances

  • Carbohydrates
  • Lectins
  • Ligands
  • Molecular Probes