Prostate cell lines secrete IL-8 in response to Mycoplasma hominis through Toll-like receptor 2-mediated mechanism

Prostate. 2006 Mar 1;66(4):386-91. doi: 10.1002/pros.20358.

Abstract

Background: The roles of Toll-like receptor 2 (TLR2) and Mycoplasma in prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2-mediated mechanism in prostate cancer cell line.

Methods: Cell surface expression of TLR2 on PC-3 cells were examined by flowcytometry. PC-3 cells were stimulated with Mycoplasma hominis (M. hominis), and IL-8 secretion and NF-kappaB activation were examined.

Results: PC-3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL-8 secretion from PC-3 cells and NF-kappaB activation in a concentration-dependent manner. Transient transfection of the dominant negative mutant TLR2(P681H) into PC-3 cells attenuated M. hominis-induced IL-8 secretion and NF-kappaB activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis-induced IL-8 secretion from the prostate cell lines including PC-3, PrEC, and transformed myofibroblasts.

Conclusions: These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2-mediated mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flow Cytometry
  • Gene Expression Profiling
  • Humans
  • Inflammation
  • Interleukin-8 / metabolism*
  • Male
  • Mycoplasma hominis / pathogenicity*
  • NF-kappa B / physiology
  • Prostate / cytology*
  • Prostate / pathology*
  • Toll-Like Receptor 2 / physiology*

Substances

  • Interleukin-8
  • NF-kappa B
  • TLR2 protein, human
  • Toll-Like Receptor 2