TEL/ARG induces cytoskeletal abnormalities in 293T cells

Cancer Lett. 2006 Sep 8;241(1):79-86. doi: 10.1016/j.canlet.2005.10.017. Epub 2005 Nov 28.

Abstract

We previously identified TEL/ARG as a novel fusion transcript consisting of the oligomerization domain of TEL and the kinase domain of ARG, in a case of acute myeloid leukemia. We report here the existence of an alternatively spliced TEL/ARG transcript lacking part of a F-actin binding domain of ARG, and the phenotype of TEL/ARG expressing 293T cells. In 293T cells, both TEL/ARG forms co-localized with the cellular beta-actin and were associated with a morphologic change of the cells, consisting in cell rounding and detachment from the tissue culture plastic. We identified the Rho inhibitor p190RhoGAP, a critical regulator of cellular adhesion, as a target of the aberrant kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Base Sequence
  • Cell Line
  • Cytoskeleton / metabolism*
  • DNA Primers
  • ETS Translocation Variant 6 Protein
  • Guanine Nucleotide Exchange Factors / metabolism
  • Humans
  • Phosphorylation
  • Proto-Oncogene Proteins c-ets / genetics
  • Proto-Oncogene Proteins c-ets / metabolism
  • Proto-Oncogene Proteins c-ets / physiology*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Repressor Proteins / physiology*
  • Subcellular Fractions / metabolism

Substances

  • ARHGAP35 protein, human
  • DNA Primers
  • Guanine Nucleotide Exchange Factors
  • Proto-Oncogene Proteins c-ets
  • Repressor Proteins