In natural populations of common wheat landrace, there has a phKL gene promoting homoeologous pairing of wheat-alien hybrids. In this study, the effects were compared among phKL, ph1b, ph2a and ph2b on homoeologous pairing of wheat-alien hybrids. The effects were indicated as ph1b > phKL > ph2b > ph2a, i. e. phKL gene was situated between gene mutants of Ph1 and Ph2.