Screening of twenty five large volume parenterals including dextrose, electrolytes, mannitol, metronidazole infusions, haemodialysis solution, water for injections and distilled water for the determination of pyrogen using Limulus Amebocyte Lysate (LAL) test has been carried out. Out of different preparations only one metronidazole injection exhibited positive LAL test, which was found pyrogen free with USP rabbit pyrogen test.