Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis

Janet C Patton; Gayle G Sherman; Ashraf H Coovadia; Wendy S Stevens; Tammy M Meyers

doi:10.1128/CVI.13.1.152-155.2006

Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis

Clin Vaccine Immunol. 2006 Jan;13(1):152-5. doi: 10.1128/CVI.13.1.152-155.2006.

Authors

Janet C Patton¹, Gayle G Sherman, Ashraf H Coovadia, Wendy S Stevens, Tammy M Meyers

Affiliation

¹ Wits Paediatric HIV Unit, Wits Health Consortium, University of the Witwatersrand, 7 York Rd., Parktown 2193, Johannesburg, South Africa. janet.patton@nhls.ac.za

Abstract

The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Chemical Analysis
Desiccation
Enzyme-Linked Immunosorbent Assay / economics*
Enzyme-Linked Immunosorbent Assay / methods
HIV Core Protein p24 / blood*
HIV Infections / blood
HIV Infections / diagnosis*
HIV Infections / immunology
HIV-1 / immunology*
Humans
Infant, Newborn
Neonatal Screening*
Sensitivity and Specificity

Substances

HIV Core Protein p24