Strategies for evaluating B*18 allelic diversity by sequence-based typing applied to studies of a population from Singapore and African-Americans

Tissue Antigens. 2006 Jan;67(1):66-9. doi: 10.1111/j.1399-0039.2005.00503.x.

Abstract

Strategies to resolve B*18 alleles which carry a deletion in intron 1 close to the 5' end of exon 2 relative to other HLA-B alleles or a null allele mutation in exon 1 and to resolve ambiguities among allele combinations including B*18 are described. B*18 allele frequencies from volunteer donors recruited for two hematopoietic stem cell registries show the presence of two alleles, B*180101 and B*1802, in a population from Singapore and only B*180101 in African-Americans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles*
  • Base Sequence
  • Black or African American*
  • China / ethnology
  • DNA Primers
  • Gene Frequency*
  • Genetic Variation
  • Genetics, Population
  • HLA-B Antigens / genetics*
  • HLA-B18 Antigen
  • Histocompatibility Testing*
  • Humans
  • India / ethnology
  • Malaysia / ethnology
  • Molecular Sequence Data
  • Registries
  • Sequence Alignment
  • Sequence Analysis, DNA / methods*
  • Singapore / epidemiology

Substances

  • DNA Primers
  • HLA-B Antigens
  • HLA-B18 Antigen