Use of different stains for microscopic evaluation of corneal scrapings for diagnosis of microsporidial keratitis

J Clin Microbiol. 2006 Feb;44(2):583-5. doi: 10.1128/JCM.44.2.583-585.2006.

Abstract

Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H(2)SO(4), cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzenesulfonates / metabolism
  • Cornea / parasitology*
  • Eye Infections, Parasitic / diagnosis
  • Eye Infections, Parasitic / parasitology
  • Humans
  • Hydroxides / metabolism
  • Keratitis / diagnosis*
  • Keratitis / parasitology*
  • Microsporidia / isolation & purification*
  • Microsporidiosis / diagnosis
  • Microsporidiosis / parasitology
  • Potassium Compounds / metabolism
  • Specimen Handling / methods*
  • Staining and Labeling / methods*

Substances

  • Benzenesulfonates
  • Hydroxides
  • Potassium Compounds
  • C.I. Fluorescent Brightening Agent 28
  • potassium hydroxide