A novel approach to varying the surface energy of biofunctional substrates has been developed, where surface energies are controlled by utilizing tunable nanopatterned substrates. In this study we functionalized the nanopattern with streptavidin, providing an adhesive interface for biotinylated probes. To obtain the surface energies, we applied the Johnson-Kendall-Roberts model to the adhesion-induced deformation of elastic beads. The results reveal a linear relationship between surface energy and ligand density, demonstrating the capability of this technique to adjust surface energy.