In vitro translation and expression of renal intrinsic factor-cobalamin receptor

Biochem Biophys Res Commun. 1991 Jun 14;177(2):751-6. doi: 10.1016/0006-291x(91)91852-4.

Abstract

The primary translation product of intrinsic factor (IF)-cobalamin receptor (IFCR) mRNA from rat kidney is a single polypeptide chain of Mr = 215,000. When expressed in Xenopus laevis oocytes the IFCR binding activity is expressed with mRNA of a size between 5 to 7 kb. These results suggest that IFCR mRNA transcripts are present in the renal tissue and encode a single chain, large molecular weight precursor. Furthermore, Xenopus oocytes can be used as a screening system in the expression cloning of the renal IFCR.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell-Free System / metabolism
  • Female
  • Gene Expression*
  • Intrinsic Factor / metabolism
  • Kidney / metabolism*
  • Molecular Weight
  • Oocytes / metabolism
  • Precipitin Tests
  • Protein Biosynthesis*
  • Receptors, Cell Surface / genetics*
  • Reticulocytes / metabolism
  • Xenopus laevis

Substances

  • Receptors, Cell Surface
  • intrinsic factor-cobalamin receptor
  • Intrinsic Factor