Crystallization and preliminary X-ray diffraction analysis of HML, a lectin from the red marine alga Hypnea musciformis

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt 11):997-9. doi: 10.1107/S1744309105033671. Epub 2005 Oct 20.

Abstract

HML, a lectin from the red marine alga Hypnea musciformis, defines a novel lectin family. Orthorhombic crystals of HML belonging to space group P2(1)2(1)2(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. A complete data set was collected at 2.4 A resolution. HML is the first marine alga lectin to be crystallized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algal Proteins / chemistry*
  • Amino Acid Sequence
  • Crystallization
  • Crystallography, X-Ray
  • Diffusion
  • Hydrogen-Ion Concentration
  • Lectins / chemistry*
  • Models, Statistical
  • Molecular Sequence Data
  • Rhodophyta / metabolism*
  • Sequence Homology, Amino Acid
  • Temperature
  • Ultracentrifugation
  • X-Ray Diffraction

Substances

  • Algal Proteins
  • Lectins