A simple method for assessing sample sizes in microarray experiments

BMC Bioinformatics. 2006 Mar 2:7:106. doi: 10.1186/1471-2105-7-106.

Abstract

Background: In this short article, we discuss a simple method for assessing sample size requirements in microarray experiments.

Results: Our method starts with the output from a permutation-based analysis for a set of pilot data, e.g. from the SAM package. Then for a given hypothesized mean difference and various samples sizes, we estimate the false discovery rate and false negative rate of a list of genes; these are also interpretable as per gene power and type I error. We also discuss application of our method to other kinds of response variables, for example survival outcomes.

Conclusion: Our method seems to be useful for sample size assessment in microarray experiments.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Computer Simulation
  • Models, Genetic*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Sample Size