Shotgun identification of proteins from uredospores of the bean rust Uromyces appendiculatus

Proteomics. 2006 Apr;6(8):2477-84. doi: 10.1002/pmic.200500630.

Abstract

We are interested in learning more about the proteome of Uromyces appendiculatus, the fungus that causes common bean rust. Knowledge of the proteins that differentiate life-cycle stages and distinguish infectious bodies such as uredospores, germlings, appressoria, and haustoria may be used to define host-pathogen interactions or serve as targets for chemical inhibition of the fungus. We have used 2-D nanoflowLC-MS/MS to identify more than 400 proteins from asexual uredospores. A majority of the proteins appear to have roles in protein folding or protein catabolism. We present a model by which an abundance of heat shock proteins and translation elongation factors may enhance a spore's ability to survive environmental stresses and rapidly initiate protein production upon germination.

MeSH terms

  • Amino Acid Sequence
  • Basidiomycota / metabolism*
  • Chromatography, Liquid
  • Fabaceae / microbiology
  • Fungal Proteins / chemistry
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptides / chemistry
  • Protein Folding
  • Proteomics / methods*
  • Sequence Homology, Amino Acid
  • Spores, Fungal / metabolism*

Substances

  • Fungal Proteins
  • Peptides