Generation of the novel monoclonal antibody against TLS/EWS-CHOP chimeric oncoproteins that is applicable to one of the most sensitive assays for myxoid and round cell liposarcomas

Am J Surg Pathol. 2006 Mar;30(3):351-6. doi: 10.1097/01.pas.0000194043.01104.eb.

Abstract

The fusion oncoproteins, TLS-CHOP and EWS-CHOP, are characteristic markers for myxoid and round cell liposarcomas (MLS/RCLS). Especially, the peptide sequence of 26 amino acids corresponding to the normally untranslated CHOP exon 2 and parts of exon 3 (5'-UTR) is a unique structure for these chimeric proteins. In this report, we have generated monoclonal antibodies against the unique peptide sequence of TLS/EWS-CHOP oncoproteins. These antibodies reacted with TLS-CHOP fusion protein, but not reacted with normal TLS and CHOP proteins by Western blot analysis. In addition, one of the antibodies also recognized the chimeric oncoprotein in archival paraffin-embedded tissue samples of MLS/RCLS. The oncoprotein was detectable by the antibody even in the paraffin-embedded tissue samples whose mRNAs were too degraded to be detected by a nested reverse transcription-polymerase chain reaction-based assay. Thus, the molecular assay using the novel antibody is expected to be one of the most sensitive diagnostic assays for MLS/RCLS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal* / immunology
  • Blotting, Western
  • Child
  • Female
  • Humans
  • Immunohistochemistry
  • Liposarcoma / diagnosis*
  • Male
  • Middle Aged
  • Oncogene Proteins, Fusion / genetics
  • Oncogene Proteins, Fusion / immunology*
  • Oncogene Proteins, Fusion / metabolism
  • Paraffin Embedding
  • RNA-Binding Protein EWS / genetics
  • RNA-Binding Protein EWS / immunology*
  • RNA-Binding Protein EWS / metabolism
  • RNA-Binding Protein FUS / genetics
  • RNA-Binding Protein FUS / immunology*
  • RNA-Binding Protein FUS / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Transcription Factor CHOP / genetics
  • Transcription Factor CHOP / immunology*
  • Transcription Factor CHOP / metabolism

Substances

  • Antibodies, Monoclonal
  • DDIT3 protein, human
  • Oncogene Proteins, Fusion
  • RNA-Binding Protein EWS
  • RNA-Binding Protein FUS
  • TLS-CHOP fusion protein, human
  • Transcription Factor CHOP