Identification of transposition proteins encoded by the bacterial transposon Tn7

Gene. 1991 Jul 31;104(1):125-31. doi: 10.1016/0378-1119(91)90478-t.

Abstract

The bacterial transposon, Tn7, encodes an elaborate array of transposition genes, tnsABCDE. We report here the direct identification of the TnsA, TnsB, TnsC and TnsD polypeptides by immunoblotting. Our results demonstrate that the complexity of the protein information devoted to Tn7 transposition is considerable: the aggregate molecular size of the five Tns polypeptides is about 300 kDa. We also report the sequence of the tnsA gene and of the 5' ends of tnsB and tnsD. This analysis reveals that all five tns genes are oriented in the same direction within Tn7.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.
  • Corrected and Republished Article

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Immunoblotting
  • Molecular Sequence Data
  • Molecular Weight
  • Open Reading Frames
  • Plasmids
  • Recombinant Proteins / analysis
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Recombinant Proteins

Associated data

  • GENBANK/M37391
  • GENBANK/M37392
  • GENBANK/M37393
  • GENBANK/S54966
  • GENBANK/S54968
  • GENBANK/S60803
  • GENBANK/S60807
  • GENBANK/S60809
  • GENBANK/S60812
  • GENBANK/X56411