Two new chiral EDTA-based metal chelates for weak alignment of proteins in solution

Org Lett. 2006 Mar 30;8(7):1275-8. doi: 10.1021/ol053049o.

Abstract

[structure: see text] A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disulfide bond is described. The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts. Alignment tensors for the new tags are linearly independent compared to those of previously published tags.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calmodulin / chemistry*
  • Chelating Agents / chemistry*
  • Cysteine / chemistry*
  • Edetic Acid
  • Lanthanoid Series Elements / chemistry*
  • Models, Molecular
  • Molecular Structure
  • Protein Binding
  • Proteins / chemistry*
  • Solutions

Substances

  • Calmodulin
  • Chelating Agents
  • Lanthanoid Series Elements
  • Proteins
  • Solutions
  • Edetic Acid
  • Cysteine