Allele frequencies of the STR loci included in the AmpFlSTR SGM Plus kit were determined in 1000 unrelated individuals from all regions of Norway. For the 10 autosomal STR loci the observed heterozygosity frequencies ranged from 0.766 (D16S539) to 0.885 (D2S1338). No significant deviation from Hardy-Weinberg equilibrium was observed. The exact test disequilibrium analysis revealed one departure from independence out of 45 pairwise comparisons. Calculations of theta coefficients for all 10 loci in two additional subpopulations of different geographical origin revealed an overall value of theta=0.002.