Are branched chain fatty acids the natural substrates for P450(BM3)?

Max J Cryle; Rocio D Espinoza; Sarah J Smith; Nicholas J Matovic; James J De Voss

doi:10.1039/b601202g

Are branched chain fatty acids the natural substrates for P450(BM3)?

Chem Commun (Camb). 2006 Jun 14:(22):2353-5. doi: 10.1039/b601202g. Epub 2006 Mar 30.

Authors

Max J Cryle¹, Rocio D Espinoza, Sarah J Smith, Nicholas J Matovic, James J De Voss

Affiliation

¹ Department of Chemistry, University of Queensland, St. Lucia, Brisbane, Australia 4072.

PMID: 16733577
DOI: 10.1039/b601202g

Abstract

Branched chain fatty acids are substrates for cytochrome P450(BM3) (CYP102) from Bacillus megaterium; oxidation of C15 and C17 iso and anteiso fatty acids by P450(BM3) leads to the formation of hydroxylated products that possess high levels of regiochemical and stereochemical purity.

MeSH terms

Bacillus megaterium / enzymology
Bacterial Proteins / chemistry*
Catalysis
Cytochrome P-450 Enzyme System / chemistry*
Fatty Acids / chemical synthesis
Fatty Acids / chemistry*
Hydroxylation
Mixed Function Oxygenases / chemistry*
Molecular Structure
NADPH-Ferrihemoprotein Reductase
Oxidation-Reduction
Stereoisomerism
Substrate Specificity

Substances

Bacterial Proteins
Fatty Acids
Cytochrome P-450 Enzyme System
Mixed Function Oxygenases
NADPH-Ferrihemoprotein Reductase
flavocytochrome P450 BM3 monoxygenases