A tagging-via-substrate technology for genome-wide detection and identification of farnesylated proteins

Methods Enzymol. 2006:407:629-37. doi: 10.1016/S0076-6879(05)07049-7.

Abstract

Protein farnesylation is one of the most common lipid modifications and has an important role in the regulation of various cellular functions. We have recently developed a novel proteomics strategy, designated the tagging-via-substrate (TAS) approach, for the detection and proteomic analysis of farnesylated proteins. This chapter describes the principle of TAS technology and details the method for detection and enrichment of farnesylated proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azides / chemistry
  • COS Cells
  • Chlorocebus aethiops
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Electrophoretic Mobility Shift Assay
  • HSP40 Heat-Shock Proteins / chemistry
  • Humans
  • Protein Prenylation*
  • Proteomics / methods*
  • Staining and Labeling / methods
  • Tandem Mass Spectrometry
  • ras Proteins / chemistry

Substances

  • Azides
  • DNAJA1 protein, human
  • HSP40 Heat-Shock Proteins
  • ras Proteins