Developing a small animal model of extracorporeal circulation

ASAIO J. 2006 May-Jun;52(3):296-302. doi: 10.1097/01.mat.0000208989.91587.f2.

Abstract

To identify nonthrombogenic devices to be used in extracorporeal circulation (ECC), an efficient, small animal model is required. Initially, a venovenous (VV) model in rabbits was designed for this purpose and was a good representation of ECC. Technical difficulties in the VV model led to the development of a more simplistic arteriovenous (AV) model. Anesthetized, tracheotomized, 3-kg rabbits were used for both models. Circuits were constructed of PVC tubing. The VV model used 8-Fr umbilical artery catheters for both drainage and reinfusion, and the AV model used a 14-GA angiocatheter for carotid artery access and a 10-Fr thoracic catheter for venous access. The AV model included a chamber to mimic oxygenator or filter modeling. Hourly measurements included blood gases, platelet counts, and fibrinogen levels for the 4-hour studies. The VV ECC groups demonstrated platelet consumption like that seen in the clinical arena. The AV model demonstrated the same with or without additional surface area within the chamber. The AV model was deemed to be superior due to its simplicity, ability for filter modeling, and decrease in intensive monitoring. However, both models are excellent designs for nonthrombogenic surface testing.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Arteriovenous Shunt, Surgical / methods*
  • Blood Gas Analysis
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism
  • Blood Platelets / ultrastructure
  • Carotid Arteries / surgery
  • Coated Materials, Biocompatible / pharmacology
  • Extracorporeal Circulation / instrumentation*
  • Extracorporeal Circulation / methods
  • Fibrinogen / analysis
  • Fibrinogen / drug effects
  • Fibrinogen / metabolism
  • Fibrinogen / ultrastructure
  • Hemodynamics / drug effects
  • Iodine Radioisotopes
  • Jugular Veins / surgery
  • Models, Animal*
  • Platelet Activation / drug effects
  • Platelet Count
  • Rabbits
  • Survival Analysis
  • Thrombosis / prevention & control
  • Time Factors
  • Tracheotomy

Substances

  • Coated Materials, Biocompatible
  • Iodine Radioisotopes
  • Fibrinogen