A single nucleotide substitution in the D domain of estrogen receptor cDNA causes amino acid alteration from Glu-279 to Lys-279 in a murine transformed Leydig cell line (B-1 F)

J Steroid Biochem Mol Biol. 1991 Jul;39(1):1-4. doi: 10.1016/0960-0760(91)90004-o.

Abstract

A polymerase chain reaction method was carried out to address the possible presence of estrogen receptor (ER) mutations in murine transformed cell lines. The segment of ER cDNA coding the C and D domains was amplified and cloned into pUC 19. Mammary carcinoma cell line (SC-3) did not show any mutation in this segment. However, the sequence analysis of ER in the Leydig cell line (B-1 F) revealed a single base change at acidic Glu-279 (GAA) to basic Lys-279 (AAA) compared with murine uterus ER cDNA. The biological significance of this mutation is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line, Transformed
  • Chickens
  • Glutamates / genetics*
  • Glutamic Acid
  • Humans
  • Leydig Cells
  • Lysine / genetics*
  • Male
  • Mammary Neoplasms, Experimental
  • Mice
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction
  • RNA, Messenger / chemistry
  • Rats
  • Receptors, Estrogen / chemistry
  • Receptors, Estrogen / genetics*
  • Tumor Cells, Cultured

Substances

  • Glutamates
  • RNA, Messenger
  • Receptors, Estrogen
  • Glutamic Acid
  • Lysine