SEN virus is a recently discovered DNA virus, and eight genotypes (A to H) were detected by phylogenetic analysis. Genotype D (SENV-D) and H (SENV-H) are thought to be associated in the etiology of post-transfusion hepatitis. Although no strong association was revealed between liver pathology and SEN virus, the viral replication in hepatocytes and transmission by blood transfusions were well characterized. The aim of this study was to investigate the prevalence of SENV-D and SENV-H in blood donors. One hundred serum samples were included in the study which were obtained from blood donors comprised of 98 male and 2 female with a mean age 36.4 years who were enrolled at Mersin University Faculty of Medicine blood center. The DNAs of SENV-D and SENV-H were detected with polymerase chain reaction (PCR), by using D10S/L2AS and C5S/L2AS primers, respectively. SEN virus DNA positivity was detected in 25 of 100 (25%) sera, of which 10 (10%) were positive for SENV-D and 15 (15%) were positive for SENV-H DNA. Although, the number of samples were not sufficient to reflect the general prevalence, our study relatively reveals that asymptomatic carriage rate of these viruses were 25% in our province.