Molecular aspects of human FcgammaR interactions with IgG: functional and therapeutic consequences

Immunol Lett. 2006 Aug 15;106(2):111-8. doi: 10.1016/j.imlet.2006.05.009. Epub 2006 Jun 12.

Abstract

The binding of IgG antibodies to receptors for the Fc region of IgG (FcgammaR) is a critical step for the initiation and/or the control of effector immune functions once immune complexes have been formed. Site-directed and random mutagenesis as well as domain-swapping, NMR and X-ray cristallography have made it possible to get detailed insights in the molecular mechanisms that govern IgG/FcgammaR interactions and to define some of the structural determinants that impact IgG binding to the various FcgammaR. It has demonstrated the role of particular stretches and individual residues located in the lower hinge region of the CH2 domain and in the CH2 and CH3 domains of the Fc region. The importance of the sugar components linked to asparagine 297 in the binding properties of IgG1, the human IgG isotype the most widely used in antibody-based therapies, has been also highlighted. These data have led to the engineering of a new generation of monoclonal antibodies for therapeutic use with optimized effector functions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use
  • Humans
  • Immunoglobulin Fc Fragments / genetics
  • Immunoglobulin Fc Fragments / immunology*
  • Immunoglobulin Fc Fragments / therapeutic use
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology*
  • Immunoglobulin G / therapeutic use
  • Mutagenesis, Site-Directed / methods
  • Protein Binding / genetics
  • Protein Binding / immunology
  • Receptors, Fc / genetics
  • Receptors, Fc / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Receptors, Fc